Pufferfish are enemies encountered in Crash Bandicoot 3: Warped, Crash Bandicoot: The Huge Adventure, and the N. Sane Trilogy remaster of the third game, in the underwater levels. In Warped, if Crash gets hit by one when it is inflated or fails to spin them away when they are deflated, it will blow helium into Crash and he will blow up like a balloon on occasion.

Originally, the pufferfish used to be purple instead of red. This can be noticed from the promotional trailer that was released before the game was nearly finished, and from the leaked beta version of Under Pressure.

One of the possible reasons of why its color was changed from purple to red is because it blended with the dark blue background of the level and it made a bit more difficult for the players to assess their attack.

Three pufferfish from The Huge Adventure appear on a trading card in Crash Bandicoot Purple: Ripto's Rampage.