N. Brio



Dr Nitrus Brio(also known as N. Brio, or plainly Brio) is an evil scientist and chemist who used to work for Dr. Neo Cortex. He first worked with Cortex in order to destroy Crash. He attemded  Madame Amberly's Academy of Evil along side N.Gin and Cortex. He was also the creator of the first fully functional Evolvo-Ray. His latest appearance was in Crash: Mind Over Mutant.

Early Years
N.Brio made his first appearance in Crash Bandicoot, where he aids Cortex in forming the Cortex Commandos. Little did they know that they would soon be trying to destroy one of there creations. He was the second last boss of the game, where he attacked Crash by throwing chemicals (possibly mutangen) in beackers  at him. He then used the mutagen on himself, transforming him into a Hulk-like monster known as Green Monster Brio. Crash eventually defeated him. He then fled Cortex Castle, and his exploding chemicals were most likely the cause of the fire. In the credits, it is said that he left to tend bars, but since that information is non cannon, it is belived that he left because he hated Cortex.

Life After Cortex
However he soon got wind of Cortex's plan to use crystals to take over the planet using the new and improved Cortex Vortex. He is arguably the main antagonist of Crash Bandicoot 2: Cortex Strikes Back, since even though he tried to get Crash Bandicoot to gather the Gems after he was sent to gather the crystals by Cortex, he was still responsible for most of the enemies in the game that stood in his way, including Tiny Tiger, Ripper Roo, and the Komodo Bros.. He then appeared again in Crash Bash, working under the instructions of Uka Uka. This was Brio's first and only playable appearence. He was then not seen again until Crash Twinsanity.



Alliance
In Crash Twinsanity, Brio returned, working with Dr. Nefarious Tropy and Dr. N. Gin. After hearing from Dingodile that the Evil Twins, Victor and Moritz, were in possesstion of a treasure, they joined forces in order to obtain the riches. Crash confronts them near the Iceberg Lab, where Brio and Dr. N. Tropy attempt to interrogate him. When that didn't work, they resort to attacking him. This plan fails as well, despite N. Brio using his new "Frog Beast" mutagen. They did end up locating the Treasure Vault beforeCortex, but were attacked by Spyro the Dragon.

NVasion
I, was, in, the first, GAAAAAAME!- N.Brio N. Brio soon appeared again in Crash: Mind Over Mutant. He and Cortex decided to work together once more. He did not appear as a boss in this game, but was the one in charge of the "Recycling Program", that was taking place at the Junkyard. His minions in this game were the Doom Monkeys, who had abandoned N.Gin. He was the ordering Crunch around, and was also the one who triggered the Crunch boss battle. Brio claims to invented many things in this game, including recycling, the Evolvo-Ray, mutagen, the bible, the heroes of the world, slinkies, the Space Head, endings and the design of NVs. After the fight against Crunch, he fled Wumpa Island, and it is unknown were he went. Many fans hope for him to reappear in the next Crash game.

Trivia

 * Although Brio's potion beakers were items in CTR, Brio himself was not present at all. Thus, he, along with Koala Kong, Komodo Moe and Tawna are the only known characters of the Crash universe before Crash Bash that were not playable characters in CTR.